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2 edition of Metabolism of selenite by rabbit erythrocytes in vitro found in the catalog.

Metabolism of selenite by rabbit erythrocytes in vitro

Marla Marie Engelhard

Metabolism of selenite by rabbit erythrocytes in vitro

by Marla Marie Engelhard

  • 51 Want to read
  • 10 Currently reading

Published .
Written in English


Edition Notes

Statementby Marla Marie Engelhard.
SeriesMaster"s theses (State University of New York at Binghamton) -- no. 694
The Physical Object
Paginationix, 69 leaves :
Number of Pages69
ID Numbers
Open LibraryOL22207252M

Compare Rabbit Red Blood Cells from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Mode of in vitro interaction of mercuric mercury with selenite to form high-molecular weight substance in rabbit blood. Chemico-Biological Interactions , 43 (3), DOI: /(83) Suzanne H. Weissman, Richard G. Cuddihy, Michele A. by:

Selenium content in tissues and meat quality in rabbits fed selenium yeast Article (PDF Available) in Czech Journal of Animal Science 52(6) June with Reads How we measure 'reads'. Phenylhydrazine-induced lipid peroxidation of red blood cells in vitro and in vivo: Monitoring by the production of volatile hydrocarbons. Biochemical Pharmacology , 33 (11), DOI: /(84) P. van by:

  Purchase Animal Cell Technology - 1st Edition. Print Book & E-Book. ISBN , Book Edition: 1. Human heterophile antibody to rabbit erythrocytes 13 to Fab fragment of human IgG6), or 50 pI of fold diluted peroxidase-conjugated Fab fragment of goat IgG antibody to Y chain of human IgG (Medical Biological Lab., Japan). The reaction was allowed to stand at 37°C for 2 h, and then pI of the substrate solution, mM p-nitrophenyl phosphate sodium salt and 1mM MgCl2 in.


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Metabolism of selenite by rabbit erythrocytes in vitro by Marla Marie Engelhard Download PDF EPUB FB2

The pathway of selenite metabolism in animals leading to the formation of methylated selenides is believed to be via the H2Se intermediate [ 6,7 ]. Selenite is rapidly taken up by erythrocytes in vitro [8,9] and metaboliz- ed by a glutathione-dependent system Cited by: The metabolism of selenite by intact rat erythrocytes in vitro.

Gasiewicz TA, Smith JC. 75Se-labeled selenite was used to study its metabolism by intact rat erythrocytes in vitro.

Utilizing both N-ethylmaleimide and excess selenite to lower erythrocyte GSH concentrations it was shown that the uptake and subsequent metabolism of selenite was Cited by: Abstract: The transfer of selenite via the erythrocytes to the plasma was investigated using Se 75 ‐bodium selenite and cow blood.

The selenite was initially fluxed into the erythrocytes by a cyanide sensitive mechanism. During the uptake period (within 1 min.) the erythrocytes consumed oxygen and it can be concluded that selenium is reduced and catalyzes the electron flow to the by: This is also reflected in the uptake of selenite by erythrocytes.

Erythrocytes from selenium-deficient sheep will take up a larger amount of radioactive selenium during in vitro incubation than will those from animals with adequate selenium (Wright and Bell, ; Weswig et al., ).

The binding of selenite, the form of selenium used in the treatment of Keshan disease, to plasma proteins and the role of Metabolism of selenite by rabbit erythrocytes in vitro book in this process have been studied. The experiments were carried out by incubating 75 Se as selenite with plasma and whole blood in vitro (human and rat) and in vivo (rat) and subsequent fractionation by Sephadex G gel by: 9.

The incorporation can be carried out after internalization of selenite by the erythrocytes in a fast, temperature dependent process.

Hemoglobin, being the major binding protein for the newly reduced selenium in the erythrocyte, might have a role in the uptake of selenite by by: 9. Rat erythrocytes were incubated in vitro with various selenium compounds at 37°.

Hemolysis occurred with some selenium compounds but not with corresponding sulfur analogues. Selenite induced more rapid loss of intracellular glutathione (GSH) than did selenocystine but was less by: 1.

Accumulation of noradrenaline (NA), 5-hydroxytryptamine (5HT) and tyramine by rabbit erythrocytes was measured at 37 degrees C in vitro.

Of the amines used only NA was broken down during incubation. This was a result of intracellular catechol-O-methyl transferase activity. by: The erythrocyte and plasma Se levels were significantly higher in the monkeys given SeMet than in those receiving selenite, but there were no differences in the GPX levels between these groups.

Naganuma, N. ImuraMode of in vitro interaction of mercuric mercury with selenite to form high-molecular weight substance in rabbit blood Chem.–Biol. Interact., 43 (), pp. Google ScholarCited by:   They erythrocyte metabolism needs ATP as a source of energy and NADH and NADPH cofactors.

The erythrocyte does not synthesize nucleic acids but it has a small requirement for ribose to synthesize nucleosides for energy transfer The metabolic needs of erythrocytes are met by metabolism of glucose through three pathways glycolysis, the hexose.

Studies on peroxidative hemolysis and erythrocyte fatty acids in the rabbit. Effect of dietary PU FA and vitamin E, J. Nutr. – PubMed A., and Yano, J., Metabolism of 75 Se-selenite by human whole blood in vitro, Can. Biochem. 47 Metabolism of selenium in the mammalian organism, J.

Agr. Food Chem. Cited by: ALLISON AC, BURN GP. Enzyme activity as a function of age in the human erythrocyte. Br J Haematol. Jul; 1 (3)– LOWY BA, RAMOT B, LONDON IM. Adenosine triphosphate metabolism in the rabbit erythrocyte in vivo and in vitro. Ann N Y Acad Sci.

Oct 13; 75 (1)– YOUNG LE, PLATZER RF, ERVIN DM, IZZO by: This is supported by the fact that mercury and selenium in HMWS found either in plasma or erythrocytes could hardly be transported through erythrocyte membrane to the other fraction (Table II and III).

Gasiewicz and Smith [32,41] suggested that the product of selenite metabolism in rat erythrocytes might be HZSe or a similarly reduced selenide compound, and could interact with cadmium or mercuric mercury Cited by: Abstract.

Inorganic forms of selenium are readily metabolized to a variety of or-ganoselenium compounds in microorganisms, plants, and animals. The metabolism of selenium may either enhance or reduce the biological activity of this by: Seko Y () Hemolysis of mouse erythrocytes by methylmercury and selenite in vitro.

Decrease in sulfhydryl concentration and lipid peroxidation by selenite. Teikyo Med J 9: – (in Japanese with English abstract) Google ScholarCited by: The erythrocyte, commonly known as a red blood cell (or RBC), is by far the most common formed element: A single drop of blood contains millions of erythrocytes and just thousands of ically, males have about million erythrocytes per microliter (µL) of blood, and females have approximately million per fact, erythrocytes are estimated to make up about.

The metabolism of selenite by intact rat erythrocytes in vitro. Chemico-Biological Interactions21 (), DOI: /(78) Ng, John W. Anderson. Synthesis of selenocysteine by cysteine synthases from selenium accumulator and non-accumulator by: Erythrocyte Membrane: Structure, Function, and Pathophysiology J.

SMITH Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan, KS Erythrocytes are unique among mammalian cells.

They contain no nucleus or subcellular metabolic structures, yet they survive for 3 to 7 rn~nths.~' During. Unfortunately, this book can't be printed from the OpenBook.

If you need to print pages from this book, we recommend downloading it as a PDF. Visit to get more information about this book, to buy it in print, or to download it as a free PDF. Methods in Immunology and Immunochemistry, Volume V: Antigen-Antibody Reactions In Vivo deals primarily with immune phenomena in tissues or in cell preparations.

This book covers a variety of topics, including anaphylaxis, tolerance, immune suppression with chemical agents, radiation effects, antibody synthesis in vitro, immunological methods Book Edition: 1.The behaviors of inorganic mercury and selenite in rabbit blood, added in vitro individually or in combination, were investigated.

Inorganic mercury incorporated into erythrocyte in the absence of selenite was mainly present in the fractions which were eluted from Cited by: Immediately after selenite has entered the red blood cells, it is reduced to selenide by cellular glutathione [52] or through the actions of thioredoxin reductase [53].

Thereafter.